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Pretreatment of Sample Before Fermentation

This document discusses pretreatment of samples before fermentation. It notes that yeast grows best at temperatures between 20-30°C and at a pH range of 4 to 4.5. It then outlines an experiment to preheat samples at different temperatures from 60-180°C for 20 minutes each, as well as heating samples at 120°C for varying times between 5-60 minutes, to determine the optimal pretreatment conditions for fermentation.

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31 views1 page

Pretreatment of Sample Before Fermentation

This document discusses pretreatment of samples before fermentation. It notes that yeast grows best at temperatures between 20-30°C and at a pH range of 4 to 4.5. It then outlines an experiment to preheat samples at different temperatures from 60-180°C for 20 minutes each, as well as heating samples at 120°C for varying times between 5-60 minutes, to determine the optimal pretreatment conditions for fermentation.

Uploaded by

ေအာင္ ေက်ာ္ စြာ
Copyright
© © All Rights Reserved
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Pretreatment of sample before

Fermentation

















Because yeast active at the temperature 0 to 50C but the optimum temperature is 20 to 30C,
Yeast can grow in a pH range of 4 to 4.5

Sample : H2O
1 : 3
Cool
Measure pH
Change pretreatment temperature Change pretreatment time
60 C for 20 min
80 C for 20 min
100 C for 20 min
120 C for 20 min
140 C for 20 min
160 C for 20 min
180 C for 20 min




120 C for 5 min
120 C for 10 min
120 C for 20 min
120 C for 30 min
120 C for 40 min
120 C for 50 min
120 C for 60 min

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