Course information form

Note to applicant: This form needs to be completed, certified and uploaded as part of your
online application. Please make sure you upload this form to the online portal as one
document.

The below named applicant has applied to register with the Health and Care Professions Council
(HCPC), which would enable them to practise within the UK. We need to obtain details of each
applicant’s professional training. Please provide details on the content of each part of the programme
that the applicant undertook at your institution.

We require content of academic and clinical training, including approximate numbers of hours within
each part of the course and the assessment methods used. Any scores obtained under examination
may also be useful. A list of procedures undertaken and departments attended during the course is
valuable in the assessment process.

Please indicate the range and scope of clinical placements undertaken. It is likely that this will take
up several pages. A syllabus is unlikely to provide sufficient applicant specific detail for registration
purposes, but a copy may be provided in addition to the Course Information form. If part of the
applicant’s training took place at another institution, please indicate N in the “Undertaken at this
institution” box. A Curriculum Certifying Document may be submitted if the detail below is included.

This form must bear the stamp or seal of the university or training institution and include contact details for the course
administrator or another appropriate member of staff who may be contacted as part of the verification process.

Name of applicant: Pooja Nichal Balan

Name of institution: Presentation College of Applied Sciences

Institution address: Manancherikunnu, Puthenvelikara, Ernakulam District, Kerala 683594, India

Institution email: presentationcollege2002@gmail.com; pcas@presentationcollege.ac.in

Institution telephone no: +91 85898 55675 +91 90375 25400

Title of professional course: Bsc In Microbiology

Institute Seal or
Date course commenced: 8th August 2010
Stamp
Date course completed: 26th June 2013

1
Please input your response into the table below. The fields will grow to accommodate your answer

Course Subject, Content and examination method, hours studied. This Assessment Undertaken
Year descriptive title of may be taken f rom the syllabus, but must only include Method. at this
1,2,3,4, subject, session, the components of the course undertaken by the Verbal = V institution
theme or module named applicant. Optional courses not undertaken by Written = W Y/N
name. the applicant should not be included. Practical = P
1 Common1 English-5H W Y

MB1B01U 72 Hours
Fundamentals of W Y
Microbiology • Unit 1- Def inition, Scope and history of
microbiology. Benef icial and harmf ul
microbes. Diversity of microbial world.
Principles of classif ication- classif ication of
bacteria & algae. Ultrastructure of
prokaryotic cell. Dif f erentiate between
eukaryote and prokaryote. Dif f erences
between archaebacteria and eubacteria.

• Unit 2- Microscopes, Staining techniques,

Culture media and culture methods (including
anaerobic bacteria) Isolation of pure cultures.

• Unit 3- Sterilisation and disinf ection physical

and chemical methods. Antimicrobial agents
mode of action

• Unit 4- Nutrition, nutritional types, growth

requirements and conditions inf luencing
growth, growth curve, growth kinetics, phases
of growth, cell division, sporulation,
germination. Enumeration & quantitation o f
bacteria and microbes. Preservation and
transport of bacteria.

W Y
MB1B02U 72 Hours
Microbial • Unit 1 : Bacterial metabolism, enzymes,
Metabolism & f actors af f ecting enzyme activity, transition
Microbial Genetics state in enzyme catalysed reactions, high
energy compounds-ATP, GTP, role of
reducing power of NAD, NADPH.

• Unit 2 : Carbohydrate metabolism,

glycolysis, alcoholic f ermentation, TCA
cycle, glyoxalate cycle, electron transport
chain, substrate level and oxidative
phosphorylation, pentose phosphate pathway.
Photosynthesis , nitrogen f ixation .
Transamination and methanogenesis.

• Unit 3: Bacterial chromosome- structure,

replication, extrachromosomal genetic
material. Plasmids - structure, replication,

2
 incompatibility, plasmid amplif ication.
Transposons -types, transposition.

Genetic exchange -conjugation,

transf ormation, transduction. Mechanism and
spread of antibiotic resistance in bacteria.

• Unit 4: DNA repair & restriction, types of

repair systems. Gene expression �
transcription, translation, control of gene
expression in microbes. Mutation-
spontaneous, induced, base pair changes,
f rame shif t, deletion, inversion, insertion,
tandem duplications, usef ul phenotypes
(auxotrophic, conditional, lethal, resistant)
Reversion vs suppression, Ames test.

Microbiology 72 hours
Practical 1
• Study the parts and usage of a Compound P Y
Microscope.
• Study the parts and working and uses of :
Autoclaves
Hot air oven
Membrane Filter
Saf ety Cabinet
Anaerobic Jar
Centrif uge
Incubator
• Preparation of culture Media (NB, NA, MA)
and dispensing media in test tubes, bottles,
petridishes.
• Cultivation of Bacteria on nutrient Agar f or
obtaining isolated colonies. Study of cultural
colony characters- Size, shape, colour etc.
• Viable Count of bacteria by pour plate/ spread
plate method.
• Examination of wet f ilms under high power
objectives.
• Preparation and examination of Hanging drop
mount f or studying the motility of bacteria.
• Preparation of slide smears f or staining.
• Staining- Principle & techniques
- Simple staining
- Gram Stainin
- Negative Staining.
- Special Staining – endospores, volutin granules
• Microscopic study of Bacteria – Cocci, Bacilli
• Preparation of Fungal media
• Cultivation of f ungi study of colony characters
of yeast and mold.

Complementary-
Biochemistry-1 W&P Y
Biotechnology-1

3
 72 Hours
Immunology • Unit 1-History of Immunology, Introduction
to Inf ection, Types of Immunity- innate W Y
immunity and acquired immunity, Cells and
organs involved in immune system

• Unit 2- Antigens- types, properties, Haptens,

Adjuvants; Vaccines - Types - Toxoids -
antitoxins., Immunoglobulins- Structure,
types and properties. Complement- structure
properties, f unction of complement
components and pathways, Major
histocompatibility complex- HLA, H2

Antigen antibody reaction- precipitation reactions,

agglutination reactions, complement f ixation,
Neutralisation reactions, Inmiunof luroscence,
ELISA, RIA.

• Unit 3- Humoral Immune response- Theories

of antibody production, plasma cells and
antibody secretion, Monoclonal antibodies,
Cell mediated immune response- Cytokines,
natural killer cells and antibody dependent cell
mediated cytotoxicity

• Unit 4- Immunohaematology- Blood groups.

Blood transf usion- Rh incompatibilities,
Hypersensitivity Reactions- Type I, II, III, &
IV. Brief of Transplantation Immunology,
Autoimmunity, Tumor Immunology

Science
Methodology and 72 Hours
Biostatistics W Y
• Unit 1- Science and Tools of Science
Types of knowledge: practical, theoretical and
scientif ic knowledge. Basis f or Scientif ic laws and
f actual truths. Revolutions in Science and
Technology.

Hypothesis; theories and laws in Science;

observations, evidences and proof s. Signif icance of
peer Review.

• Unit 2-- Experimentation in Science and Data

Handling Design of an experiment;
experimentation; observation; data collection;
interpretation and deduction. Necessity of
units and dimensions; repeatability and
replication. Documentation of experiments,
Record keeping. Connection between
measurements and underlying
theory.Documentation of experiments.
Nature and types of data - typical examples; data
acquisition; treatment of data; data interpretation.
Signif icance of statistical tools in data
interpretation: graphs, tables, histograms and pi
diagrams.
Statistical testing of hypothesis, null hypothesis.
Signif icance test - Statistics based acceptance or
rejection of a hypothesis. Deduction of scientif ic
correlation, patterns and trends.

4
 • Unit 3- Nature and scope of statical methods
and their limitation.
compilation, classif ication,tabulation, and application
in lif e science.
Graphical representation,Measure of average and
dispersion mean,medium,mode.

• Unit 4- Sampling methods - simple random,

stratif ied, systematic and cluster sampling
procedures. Sampling distribution, Probability,
Tests of signif icance
based on T, Chi-square and F Test Designing and
methodology of experiment

Microbiology 72 Hours
Practical � II P Y
• Slide agglutination test ,Blood grouping, ASO
Bacterial agglutination test
• Precipitation reaction � RPR ,VDRL, , ODD,
RID,
• ELISA-Demonstration-Titration of Antibody ,
Widal Test.

Complementary W&P Y
course-
-Biochemistry-
Elemetal
biochemistry

-Biotechnology-
Molecular biology
and recombinant
DNA technology

2 Industrial 90 Hours W Y
Microbiology • Unit I: General concepts of industrial
microbiology, principles of exploitation of
microorganisms of their products.
Introduction to microbial products obtained
by industrial process-primary and
secondary metabolites. Industrial strains�
characteristics and isolation techniques-
primary and secondary staining techniques,
strain improvement.
.

• Unit 2: Industrial f ermentation equipment

and its uses, types of fermentation -single,
batch, continuous, dual or multiple, solid -
state and submerged f ermentation.
Fermentation media f ormulation strategies,
means of providing energy, carbon,
nitrogen, vitamin and mineral sources, role
of buf fers, precursors, inhibitors, inducers and
antif oams, products recovery and purif ication.

• Unit 3 : Industrial products derived f rom

microbes, industrial Enzymes-amylase,
proteinase, celluase, Aminoacid production
- glutamic acid and lysine. Production of
antibiotics penicillin, streptomycins, Vitamins-

5
 ribof alvin, cyanocobalamin. Vaccines - genetic
recombinant vaccines.

• Unit 4: Immobilization methods, adsorption;

covalent linkages, membrane entrapment-
advantages and disadvantages, Intellectural
Property Rights (IPR) IPR - Def inition - WTO -
Def mition - Functions - Forms of IPR
Protection.

Soil microbiology
72 hours W Y
• Unit 1-Soil environment- soil structure,
physico chemical characteristics, microbial
composition and inf luence of soil and
environmental f actors on soil micro f lora
.Quantitative and qualitative estimation of
microorganisms in soil. Organic matter
decomposition, Methanogenesis Humus
f ormation.

• Unit 2-Major biogeochemical cycles and the

organisms. Microbial transf ormation of
carbon-nitrogen-phosphorus and sulphur.
Microbes in composting and biogas
production

• Unit 3- Microorganisms in soil

f ertility.Rhizosphere concept,R:S ratio
,Interaction between plant and rhizosphere
f lora.Growth promoting rhizobacteria,soil
enzymes,
Biocheleters(siderophore).Bif ertilizers-Types
and importance

• Unit 4- Biological nitrogen f ixation -

Nitrogenase enzyme-nif genes-symbiotic
nitrogen f ixation (Rhizobia,Frankia)-non
symbiotic nitrogen f ixation
(azotobacter,Azospirillum). VAM-ecto-endo-
ectendomycorrhizae.

Food Microbiology
72 Hours
• Unit I - History of f ood microbiology. Food W Y
as a substrate f or micro organisms -.
Micro organisms important in f ood
microbiology; Molds, yeasts and bacteria -
General Characteristics - Classif ication and
importance. Principles of f ood preservation -
Asepsis - Removal of micro organisms,
anaerobic conditions - High temperature - Low
temperature - Drying – Food additives.

• Unit 2- Contamination and spoilage -

vegetables and f ruits, meat and meat
products, milk and milk products - f ish and
sea f ood - Poultry, Spoilage of canned f oods.
Food borne inf ections, poisoning and
intoxications Microbiological examination of
f ood, Milk. HACCP- def inition and principles
(brief )

• Unit 3- Importance of microbes in f ood

industries. Fermented f ood products by

6
 microbes -Bread, Vinegar etc. Alcoholic
beverages- wine, beer, cedar. Oriental
f ermented f oods. Fermented vegetables. Milk
and milk products- butter, cheese, Probiotics
(brief study)

• Unit 4- Microbial cells as f ood-single cell

proteins- Bakers yeast Edible mushroom-
types and production ,Enzymes in f ood
industry
Microbiology
Practical �III 72 Hours P Y
• Enumeration of soil microbes by Plate culture
method and isolation of microorganisms f rom
soil sample - Bacteria, Fungi, Actinomycetes
and Azotobacteria.
• Isolation of cellulolytic, Amylolytic, Lipolytic
and Proteolytic Bacteria f rom Soil
• Wine Production f rom grapes
• Immobilization of yeast cells
• Study of Microbial contamination in f ood
products
• Analysis of f ood samples- Vegetables, Fruits,
Fish, Meat and processed f ood & isolation of
bacteria and f ungi.
• Milk analysis by SPC and MBRT
• Enumeration and isolation of Lactobacillus
f rom curd
• Enumeration and isolation of Bacteria and
mold f rom f ermented f oods
• Production of Alcohol f rom Cashew Apple
• Mushroom cultivation
Complementary W&P Y
course-
-Biotechnology-
plant and animal
biotechnology

-Biochemistry-
Enzymology and
metabolism 1

Aquatic 72 Hours
microbiology W Y
• Unit 1- The aquatic environment and
distribution of microorganisms in the
aquatic environment. Aquatic ecosystem-
f resh water(ponds,lakes,stream) Marine
(estuaries.mangroves,deep sea).Water
zonations-upwelling.Benthic
microorganisms.Marine microf lora and
biof ouling

• Unit 2- Techniques f or the study of aquatic

microorganisms.The role and importance of
aquatic microbial ecosystem.microbial
consortia.Surf ace attachment and biof ilm
development. Antibacterial and bioactive
compounds f rom aquatic microorganisms

• Unit 3- Water pollution, microbial changes

induced by inorganic and organic
pollutants.metals as pollutants.algal
blooms.biological and chemical control of
algal blooms
7
 • Unit 4- Potability of water.Purif ication and
disinf ection.indicator
organisms.microbiological examination of
drinking water.Water born diseases and
control measures

90 Hours

• Unit 1- Concepts of microbial ecology W Y

.Microorganisms in the natural
Environmental environment;Extreme environments and their
microbiology adaptations.Signif icance of microbial activities
in the environment

• Unit 2- Aerobiology,.Sources of
microorganisms in the air and their
quantif ication techniques. Methods of
sampling air.Factors af f ecting the extent and
type of air microf lora

• Unit 3- Biosensors and biological indicators.

Waste water management and sewage
treatment.Bioreactors f or waste
treatments.Biosurf actants.Biodeterioration of
industrial products such as textiles ,paper,
pulp,wood and paints.

• Unit 4- Bioremediation and strategies f or

bioremediation. Phytoremediation Microbial
degradation of petroleum and petroleum
products,Pesticide degradation..Biostimulation
and bioaugmentation.Fate of engineered
microorganisms in the environmentMicrobial
leaching and metal recovery.Microbes in
mining and enhanced oil recovery.

Agricultural 72 hours
microbiology W Y
• Unit 1- Brief account of microbial
interactions -symbiosis-mutualism-
commensalism-Amensalism-synergism-
parasitism-predation. Plant - microbe and
Animal-microbe interactions

• Unit 2- Microbial diseases of plants-common

bacterial, f ungal and viral plant pathogens.
Transmission and control measures. Natural
def ence mechanisms of plants. Microbial
warf are on plants

• Unit 3- Phyllosphere microf lora and its

importance.Endophytic micrf lora.
Microboilogy of wood and silage,Tobacco
curing. Role of microbes in retting Rumen
microbiology

• Unit 4- Biopesticides-Bacterial-viral-f ungal

pesticides Biological control of plant
diseases.Integrated pest management

8
 Microbiology 72 Hours
Practical � IV
• Enumeration and isolation of microorganism P Y
f rom f resh water and marine system.
• Microbial investigation of drinking water
samples, total bacteria count, colif orm
test - MPN, Membrane Filter Technique.
• Estimation of BOD.
• Study of common Plant pathogen
• Isolation of pathogen- Citrus Canker , wilt.
• Estimation of rhizosphere microbial
population and calculation of R:S ratio.
• Isolation of nitrogen f ixing bacteria -
Rhizobium, Azospirillum, Azotobacter

W&P Y
Complementary
course-

-Industrial and
environmental
biotechnology

-Biochemistry-
metabolism ii

3 Medical 90 hours W Y
Bacteriology- I • Detailed study of Morphology, Cultural
characteristics, Biochemical, Epidemiology,
Pathogenesis, Laboratory diagnosis,
Prophylaxis and Treatment of the f ollowing
bacteria:
• Unit 1: Staphylococcus, Streptococcus

• Unit 2: Pneumococcus, Neisseria

• Unit 3: Corynebacterium, Bacillus, Clostridium

• Unit 4: Enterobacteriacea I- Colif orms,

Proteus ,

Enterobacteriacea II:- Shigella, Salmonella

• Unit 5: Vibrio, Pseudomonas, Haemophilus

Medical 72 Hours W Y
Bacteriology- II

9
 Detailed study of Morphology, Cultural
characteristics, Biochemical, Pathogencity,
Epidemiology, pathogenesis, laboratory diagnosis,
prophylaxis
and treatment of the f ollowing bacteria

• Unit 1: Mycobacterium tuberculosis, M.

leprae, Atypical mycobacteria, Actinomyces

• Unit 2: Bordetella, Brucella, Yersinia,

Pasteurella, Francisella

• Unit 3: Spirochetes, Mycoplasma

• Unit 4: Rickettsiaceae, Chlamydiae

Medical Mycology W Y
72 Hours

• Unit 1: General Characters of Fungi- Yeast

and mold.Nutrition in Fungi.Cultivation of
Fungi - Culture media and cultural
characters.Methods f or isolation of f ungi.
Staining methods used in mycology - wet
mount and dif f erential staining. Study of
microscopic morphology - Ultra structure of
yeast.

• Unit 2: Reproduction in Fungi- Asexual and

Sexual method Classif ication of Fungi -
Principles and Approaches. Antif ungal agents-
Mechanism of action

• Unit 3: Fungal Diseases: Causative Fungi,

Clinical Manif estations, Laboratory Diagnosis
and treatment - Brief account. Superf icial
mycoses .Pityriasis versicolor ,
Dermatophytoses, Piedra Subcutaneous
mycoses - Mycetoma, Rhinosporidiosis,
Phycomycosis, Sporotrichosis

Medical
Parasiotology 72 hours W Y

• Unit 1: Parasitology - General Concepts -

Introduction to Parasitology, Classif ication -
Host parasite relationship.
Laboratory technique in Parasitology
Examination of f aeces f or ova and cysts - Verm
burden. Concentration methods, Floatation,
Sedimentation techniques, staining by iron
heamotoxylin methods, Blood smear examination -
thick / thin Smears - Cultivation of protozoal
parasites.

• Unit 2: Protozoology: Pathogenic

mechanisms, Disease transmissions and
their lif e cycles - Entamoeba and human
disease , Plasmodia, Leishmania,
Trypanosoma, Giardia,Trichomonas,
Balantidium, Toxoplasma, Cryptosporidium
10
 and other protozoan parasites causing human
inf ections. Inf luence of parasitic inf ections on
immunocompromised hosts.

• Unit 3- Helminthology: Classif ication,

Cestodes - Taenia. solium ,T. saginata,
T.echinococcus , trematodes - Fasciola
hepatica, Fasciolopsis buskii , Paragonimus
westermanii, Nematodes -Ascaris ,
Schistosomes, Anchylostoma, Trichuris,
Trichinella, Enterobius, Strogyloids and
Wuchereria their lif e cycle , Transmission,
pathogenicity and Lab Diagnosis.

Microbiology 90 Hours W Y
Practical � V
• General procedure f or the systematic study of
Bacteria - Morphology, Staining, Colony
Characteristics on BA ,MA, other selective
Media.
• Biochemical reactions of Bacteria: Sugar
Fermentation , IMVIC ,H2S
production,urease,Catalase, Oxidase ,TSI
• Identif ication of Bacteria- Staphylococuus,
E.coli, Klebsiella, Pseudomonas, Proteus,
Salmonella typhi, Shigella, Vibrio cholerae.
• Cultivation of Fungi- Study of colony
characters of yeasts and Molds.
• Microscopic morphology of molds- Pencillium,
Aspergillus. Mucor, Rhizopus, Fusaruim , etc
by Lactophenol cotton blue mount
examination.
• Gram staining of yeast.
• Examination of Germ tube - Candida albicans

W&P Y
Open course Computer Assembling

Medical Virology 72 hours W Y

• Unit 1: General characters of viruses -

structure, classif ication, multiplication,
cultivation. Structure and replication of
bacteriophages

• Unit 2: Characters and pathogenic

signif icance of pox viruses, herpesviruses,
picorna viruses & myxoviruses

• Unit 3: Characters and pathogenic

signif icance of arboviruses, rhabdoviruses,
hepatitis viruses, oncogenic viruses & HIV

• Unit 4: Diagnosis of viral inf ections.

Immunoprophylaxis and chemoprophylaxis of
viral inf ections.

11
Diagnostic 90 Hours
Microbiology
• Unit 1:Saf ety in Clinical Microbiology
laboratory. Laboratory associated inf ections.
Classif ication of microorganisms on the W Y
basis of hazards. Good laboratory practices.
Microbiological saf ety cabinets- Types.
Disinf ection and decontamination of
laboratory wastes. WHO saf e code of practice
f or a clinical microbiology laboratory.
Guidelines f or the collection and transport of
specimens

• Unit 2: Scheme f or the microbiological

examination of sputum, throat and mouth
specimens, nasopharyngeal swabs and
aspirates, ear discharges, eye specimens,
CSF, blood and bone marrow

• Unit 3: Microbiological examination of stool

specimens, urine, urogenital specimens, skin
specimens, pus f rom wounds, abcesses,
burns and sinuses, and ef f usions.

• Unit 4: Serological diagnosis: Applications of

serological techniques such as agglutination
reactions, precipitation reactions, complement
f ixation tests, and enzyme immunoassay f or
the diagnosis of Bacterial viral and
immunological diseasesMolecular
techniques in Microbiology- principles and
applications Hybridisation, PCR, RFLP

• Unit 5: Laboratory control of antimicrobial

therapy Antibiotic sensitivity tests. Disc
dif f usion and dilution methods,
Determination of MIC and MBC. Antibiotic
assays in body f luids.. Care and
management of laboratory animals. Legal
requirements f or animal experiments.
General aspects of organization of animal
experiments- Preparation of animals, common
experimental procedures, Humane methods
of killing animals. Handling of common
laboratory animals: Rabbit, guinea-pig, mouse
and rat

Sanitation 72 hours
Microbiology • Unit 1: General concept of sanitation and W Y
disinf ection .Sanitation of industrial and
f ood processing units Air sanitation,Saf e
location of animal houses,hospitals
,industrial f ermentation units etc based on
air sanitation.Air borne diseases and
preventive measures.Biological
weapons,their regulation and precaution.

• Unit 2: Microbiology of municipal sewage and

sewage treatment.BOD and COD Concept .
Treatment of Industrial ef f luent- Waste water
treatment-Mechanical and biological. Aerobic

12
 and anaerobic treatments.Domestic septic
tank. Treatment of municipal water
supplies.water borne diseases

• Unit 3: Solid waste disposal-sanitary land

f ills,composting,vermicompost.Disposal of
animal and agricultural waste.
Methanogenesis and biogas production

Microbiology 90 Hours
Practicals – VI P Y
Seminar • Isolation of pathogens f rom Clinical
Project Speciments
viva • Identif ication of Pathogens- Bacteria
Antimicrobial activity - Disc dif f usion & broth dilution
test, MIC
• Serological Test -WIDAL, RPR Card Test

13