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Tryptone Glucose Extract Agar (TGE Agar) : Composition

Tryptone Glucose Extract Agar (TGE Agar) is designed for the enumeration of bacteria in various samples like water and dairy products, with a composition including tryptone, meat extract, glucose, and agar. The agar is prepared by dissolving the powder in distilled water, sterilizing, and can support the growth of several bacterial species. It is important to store the prepared media below 8°C and use it for research and development purposes only.

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24 views2 pages

Tryptone Glucose Extract Agar (TGE Agar) : Composition

Tryptone Glucose Extract Agar (TGE Agar) is designed for the enumeration of bacteria in various samples like water and dairy products, with a composition including tryptone, meat extract, glucose, and agar. The agar is prepared by dissolving the powder in distilled water, sterilizing, and can support the growth of several bacterial species. It is important to store the prepared media below 8°C and use it for research and development purposes only.

Uploaded by

ngonguyenhoanguyen
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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70159 Tryptone Glucose Extract Agar (TGE Agar)

Tryptone Glucose Yeast Extract Agar is recommended for enumeration of bacteria in water, air, milk
and dairy products.

Composition:

Ingredients Grams/Litre
Tryptone 5.0
Meat extract 3.0
Glucose (Dextrose) 1.0
Agar 15.0
Final pH 7.0 +/- 0.2 at 25°C

Store prepared media below 8°C, protected from direct light. Store dehydrated powder in a dry place
in tightly-sealed containers at 2-25°C.
Appearance: Faint yellow to faint beige to faint brown colored, homogenous, free flowing
powder.
Gelling: Firm
Color and Clarity: Light yellow to light beige to light brown colored, clear to slightly opalescent gel
forms in petri plates.

Directions:
Suspend 24 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by
autoclaving at 121°C for 15 minutes. Mix well before pouring. When the dilution of the original
specimen is greater than 1 in 10, add 10 ml of sterile 10% solution of Skim Milk powder (Cat. No.
70166) per litre and do not overheat during sterilization.

Principle and Interpretation:


Tryptone and meat extract provide nitrogenous compounds and vitamin B complex. Glucose is the
energy source.

Cultural characteristics after 18-24 hours at 35-37°C.

Organisms (ATCC) Inoculum (CFU) Growth Recovery


Bacillus subtilis (6633) 50-100 +++ ≥ 70%
Enterobacter aerogenes (13048) 50-100 +++ ≥ 70%
Escherichia coli (25922) 50-100 +++ ≥ 70%
Lactobacillus casei (9595) 50-100 +++ ≥ 70%
Pseudomonas aeruginosa (27853) 50-100 +++ ≥ 70%
Staphylococcus aureus (25923) 50-100 +++ ≥ 70%
Enterococcus faecalis (29212) 50-100 +++ ≥ 70%
Streptococcus pyorogenes (27853) 50-100 +++ ≥ 70%

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References:
1. L.S. Bowers, J.G. Huker, Tech. Bull. 228, N.Y. State Agr. Exp. Sta (1935)
2. American Public Health Association, Standard Methods for the examination of Dairy
Products, 9th Ed., APHA, New York, N. Y. (1948)
3. F.P. Downes, K. Ito, (Eds.), Compendium of Methods for the Microbiological Examination of
Foods, 4th Ed., APHA, Washington, D.C. (2001)
4. H.M. Wehr, J.H. Frank, Standard Methods for the Examination of Dairy Products, 17th Ed.,
APHA Inc., Washington, D.C. (2004)
5. A.D. Eaton, L.S. Clesceri, A.W. Greenberg, (Eds.), Standard Methods for the Examination of
Water and Wastewater, 21st Ed., APHA, Washington, D.C. (2005)

Precautions and Disclaimer


This product is for R&D use only, not for drug, household, or other uses. Please consult the Material
Safety Data Sheet for information regarding hazards and safe handling practices.

The vibrant M, Millipore, and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or
its affiliates. Detailed information on trademarks is available via publicly accessible resources.
© 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

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