SAMPLE COLLECTION

Sample collection means the process by which a sample of a bodily substance is

obtained for use in the laboratory analysis to determine the presence of certain
substances, legal or illegal, in the sample. Collecting specimens is the process
of acquiring tissue or fluids for laboratory analysis. Collecting specimens is the
process of acquiring tissue or fluids for laboratory analysis. Generally, some of
the samples collected in medical laboratories may include serum samples,
virology swab samples, biopsy and necropsy tissue, cerebrospinal fluid, whole
blood for PCR, and urine samples. Medical Assistants collect these samples and
then place them in specific containers for successful processing in a lab. The
most common specimens used in clinical chemistry are blood and urine. Many
different tests exist to detect and measure almost any type of chemical
component in blood or urine. Components may include blood glucose,
electrolytes, enzymes, hormones, lipids (fats), other metabolic substances, and
proteins.

Specimen collection is a common component of routine check-up for your

health. It goes hand-in-hand with lab tests, allowing medical professionals to
obtain tissue or fluids to look for any changes in your health. In addition, they
help physicians diagnose medical conditions, plan treatments, and monitor
diseases. Mastering this skill will lead to a much better experience for
healthcare practitioners and their patients.

As you can see, specimen collection is an essential procedure used for many
medical diagnoses. This fundamental skill may not be exciting for new
healthcare professionals, but it is crucial in developing the correct diagnosis for
each patient. Therefore, it can be particularly beneficial for Medical Laboratory
Scientist for specific investigations to learn the proper method of collecting
specimens. Specimens should be collected aseptically and as soon as possible
after death. A separate sterile instrument should be used for each collection site
and each specimen should be placed in a separate sterile container in small
amounts of viral transport media or saline.

What is the Purpose of Specimen Collection?

Specimen collection is vital for acquiring an accurate and timely laboratory test
result. To properly collect specimens, they must be obtained through designated
containers, correctly labelled, and promptly transported to the lab.

How Long Does it Take to Collect Specimens?

Most specimens can be collected in a matter of minutes. It normally only takes
a few minutes to transport them to the lab as well. However, some specimen
collection may require more steps. It often depends on the type of tissue or fluid
the patient has provided.

Who Can Collect Specimens?

Nurses typically collect specimens like urine, stool, and sputum (saliva/mucus
from the respiratory tract). In addition, they may perform venipuncture to obtain
blood samples. Specimens can also be collected by a variety of other healthcare
professionals, such as Medical Assistants. Although the rules can vary by state,
Medical Assistants (MAs) are often allowed to collect specimens through non-
invasive measures. They preserve specimens for testing, including urine,
sputum, semen, and stool. This common task allows MAs to assist physicians
and other healthcare professionals by allowing them to see more patients or
perform other tasks.

Why Should ML S Learn How to Collect Specimens?

Although most facilities employ nurses or other healthcare professionals, it is a
valuable skill that can lead to greater opportunities which include avoidance of
pre-analytical errors, and errors dew to parallax. Therefore, proper training is
necessary. Once personnel are prepared to perform certain specimen collection
duties, the officer could become more indispensable to their employer and
possibly gain greater responsibilities.

Training Requirements for Specimen Collection

Requirements for specimen collection can vary and depend on your location or
profession. Aspiring nurses and other healthcare professionals especially MLS
will usually receive this training through their educational programs. However,
others may pursue a job as specimen collectors, which are usually called
Phlebotomist. Some healthcare professional schools in the developed countries
introduces courses like assistance or technicians in specimen collection,
pharmacology, medication administration, diagnostic imaging, and more.

Specimen Collection Procedure

With these steps, you can start to learn more about the process of collecting
specimens. Of course, just like any other skill, specimen collection requires a
little time and practice to get it right. Remember to follow the rules of your
facility and ask for help whenever necessary.

Patient’s preparation for Specimen Collection

While most collections don’t require much preparation, there are some
guidelines/rules that must be followed. These include reviewing the appropriate
information, such as the indicated specimen type, the volume, the procedure, the
collection materials, patient prep, and storage instructions.

Essential Materials and Equipment for Specimen Collection

Typically, the suggested supplies for specimen collection include but are not
limited to the follo wing items:
 Gloves
  Apron
 Hand sanitizer
 Specimen container
 Body material sample
 Incubator
 Refrigerator or freezer.

Some Steps in Specimen Collection

Here are some of the general guidelines you should follow when collecting
specimens from a patient:
1. Verify the patient’s identity. Some examples of acceptable identifiers
include the patient’s name, date of birth, and hospital number.
2. Acquire a sample from the patient. Treat all biological material as
potentially hazardous and follow your facility’s guidelines.
3. Process the specimen as required by your facility or employer.
4. Store the specimen. Appropriate storage is critical to maintaining the
integrity of the specimen and, therefore, the test results.

Patient’s Recovery
After the procedure, the patient can usually resume their normal activities.
There is not a recovery period for most types of specimen collection since they
are non-invasive e.g., urine, stool, etc.

Possible Risks or Complications of Specimen Collection

Specimen collection is often safe and relatively painless for patients. Although
there may be pain associated with blood inducements, it should not be
significant or cause any lasting pain and/or damage.

Professional Guidelines for Understanding the Skill of Specimen Collection

1. Avoid frequent problems with attention to detail.
2. Follow the guidelines of your facility instructions strictly.
3. Use two patient identifiers or more before collecting the sample.
4. Label containers for specimens while in the presence of the patient.
5. Ensure you aren’t using any expired materials like specimen containers,
gloves, needle and syringe etc.
6. Ensure that you are using the correct container. Some require
preservatives, while others do not.
7. Do NOT transfer/pour specimens from one type of container into another.
 8. Obtain a large enough quantity of the specimen to ensure the test can be
performed (check the test’s requirements before collecting the sample).
9. Tighten container lids to avoid any leakage or contamination.
10. Keep the specimen at the recommended temperature as specified in the
investigation requirements.
11. For urine collection, instruct the patient on how to provide a clean-catch,
midstream sample.
12. Remember to add the specified preservative in the test requirements to
the collection container before you ask for a sample.

CLEANING AND MAINTANANCE OF LABORATORY GLASSWARES

Glass is a versatile material that is used in a wide variety of applications, from
windows and mirrors to tableware and cookware. Proper care and maintenance
can help to ensure that glass lasts for many years. With proper care, glass can be
a beautiful and durable when used in your home, office or laboratories.
Principles of cleanliness vary from lab to lab depending on the type of work
carried out in a lab. Whatever standard is required, basic methods for '
cleanliness of glassware are universal. The reliability of experimental work
depends on the availability of clean glassware. For example, one would have
little confidence in the accuracy of a laboratory analysis if greasy, dirty or
contaminated glassware were used. Glassware should be cleaned immediately
after use to avoid stubborn residues. Wash glassware in hot water and rinse with
cold water. Crystal glass should be washed in lukewarm water. Scrub glassware
thoroughly with a brush ensuring that all surfaces are covered.

Types of Glass
 Soda lime glass: Used for consumable apparatus, e.g. tubing, rod, and
also for cheaper containers. It is susceptible to attack from alkaline
solutions and is unsuitable for strong heating.
 Borosilicate glass Suitable for heating e.g. Pyrex.
 Silica glass: Suitable for extreme and sudden changes of temperature. It
is very resistant to acids and does not absorb ultra violet light, thus being
useful for lenses and cuvettes.

General Maintenance of Glassware

1. Cleaning
Glassware should be cleaned immediately after use to avoid stubborn
residues. Wash glassware in hot water and rinse with cold water.
Crystal glass should be washed in lukewarm water. Scrub glassware
thoroughly with a brush ensuring that all surfaces are covered. Do not
use any harsh cleaning utensils such as steel wool pad to wash
glassware as this will cause scratches.

2. Detergents

For stubborn stains, use a mild detergent only. Always follow the
manufacturer’s instructions and do not use more than the
recommended quantities. After using a detergent, ensure that
glassware is rinsed thoroughly to avoid any detergent residues being
left on the glass.

3. Dishwashers

Glassware can be washed in the dishwasher. However, take care not to

wash glasses with very delicate long stems in the dishwasher as the
possibility exists that these may break.

4. Rotation

Avoid using scratched, cracked or chipped glasses

5. Stacking

Avoid stacking glassware unless it is specifically identified as

stackable. For best results store glasses in appropriate crates.

6. Thermal Shock

Do not expose glassware to an open element or electric plate and do

not place it in the oven. Normal glass can withstand temperature
changes of 42 degrees, and tempered glass can withstand temperature
changes of 130 degrees.

7. Microwaves

Never allow glassware to be placed inside a microwave oven

The following are some tips for caring for glass:
1. Clean glass regularly with a mild soap and water solution.
2. Avoid using abrasive cleaners or scouring pads, as these can scratch the
surface.
3. Wipe-Dry glass thoroughly after cleaning to prevent water spots.
4. Avoid cleaning glass in direct sunlight, as this can cause streaks.
5. Be careful not to place hot objects directly on glass, as this can cause
thermal shock and breakage.
6. If glass does become scratched, you can try to polish it out with a mild
abrasive cleaner or a glass polishing compound but this is not important
and acceptable in medical laboratory sciences.
Additional tips for proper caring for specific glass types:
Mirrors: Mirrors should be cleaned with a mild soap and water solution, and
then polished with a soft, lint-free cloth. Avoid using ammonia-based cleaners,
as these can damage the mirror's reflective coating.
Tempered glass: Tempered glass is a type of safety glass that is more resistant
to breakage than regular glass. However, it is still important to handle tempered
glass with care. Do not clean tempered glass with harsh chemicals or abrasive
materials.
Smart glass: Smart glass is a type of glass that can change its opacity or
transparency in response to electrical signals. Smart glass does not require as
much maintenance as regular glass, but it is important to clean it regularly with
a mild soap and water solution.

Glass is a versatile material that is used in a wide variety of applications,

from windows and
mirrors to tableware and cookware. Proper care and maintenance can help to
ensure that glass
lasts for many years. The following are some tips for caring for glass: Clean
glass regularly with
a mild soap and water solution. Avoid using abrasive cleaners or scouring
pads, as these can
scratch the surface. Dry glass wipe thoroughly after cleaning to prevent
water spots. Avoid
cleaning glass in direct sunlight, as this can cause streaks. Be careful not to
place hot objects
directly on glass, as this can cause thermal shock and breakage. If glass does
become scratched,
you can try to polish it out with a mild abrasive cleaner or a glass polishing
compound. With
proper care, glass can be a beautiful and durable addition to your home or
office. Here are some
additional tips for caring for specific types of glass: Mirrors: Mirrors should be
cleaned with a
mild soap and water solution, and then polished with a soft, lint-free
cloth. Avoid using
ammonia-based cleaners, as these can damage the mirror's reflective coating.
Tempered glass:
Tempered glass is a type of safety glass that is more resistant to
breakage than regular glass.
However, it is still important to handle tempered glass with care. Do not
clean tempered glass
with harsh chemicals or abrasive materials. Smart glass: Smart glass is a type
of glass that can
change its opacity or transparency in response to electrical signals. Smart glass
does not require
as much maintenance as regular glass, but it is important to clean it regularly
with a mild soap
and water solution
Glass is a versatile material that is used in a wide variety of applications,
from windows and
mirrors to tableware and cookware. Proper care and maintenance can help to
ensure that glass
lasts for many years. The following are some tips for caring for glass: Clean
glass regularly with
a mild soap and water solution. Avoid using abrasive cleaners or scouring
pads, as these can
scratch the surface. Dry glass wipe thoroughly after cleaning to prevent
water spots. Avoid
cleaning glass in direct sunlight, as this can cause streaks. Be careful not to
place hot objects
directly on glass, as this can cause thermal shock and breakage. If glass does
become scratched,
you can try to polish it out with a mild abrasive cleaner or a glass polishing
compound. With
proper care, glass can be a beautiful and durable addition to your home or
office. Here are some
additional tips for caring for specific types of glass: Mirrors: Mirrors should be
cleaned with a
mild soap and water solution, and then polished with a soft, lint-free
cloth. Avoid using
ammonia-based cleaners, as these can damage the mirror's reflective coating.
Tempered glass:
Tempered glass is a type of safety glass that is more resistant to
breakage than regular glass.
However, it is still important to handle tempered glass with care. Do not
clean tempered glass
with harsh chemicals or abrasive materials. Smart glass: Smart glass is a type
of glass that can
change its opacity or transparency in response to electrical signals. Smart glass
does not require
as much maintenance as regular glass, but it is important to clean it regularly
with a mild soap
and water solution
Glass is a versatile material that is used in a wide variety of applications,
from windows and
mirrors to tableware and cookware. Proper care and maintenance can help to
ensure that glass
lasts for many years. The following are some tips for caring for glass: Clean
glass regularly with
a mild soap and water solution. Avoid using abrasive cleaners or scouring
pads, as these can
scratch the surface. Dry glass wipe thoroughly after cleaning to prevent
water spots. Avoid
cleaning glass in direct sunlight, as this can cause streaks. Be careful not to
place hot objects
directly on glass, as this can cause thermal shock and breakage. If glass does
become scratched,
you can try to polish it out with a mild abrasive cleaner or a glass polishing
compound. With
proper care, glass can be a beautiful and durable addition to your home or
office. Here are some
additional tips for caring for specific types of glass: Mirrors: Mirrors should be
cleaned with a
mild soap and water solution, and then polished with a soft, lint-free
cloth. Avoid using
ammonia-based cleaners, as these can damage the mirror's reflective coating.
Tempered glass:
Tempered glass is a type of safety glass that is more resistant to
breakage than regular glass.
However, it is still important to handle tempered glass with care. Do not
clean tempered glass
with harsh chemicals or abrasive materials. Smart glass: Smart glass is a type
of glass that can
change its opacity or transparency in response to electrical signals. Smart glass
does not require
as much maintenance as regular glass, but it is important to clean it regularly
with a mild soap
and water solution
Glass is a versatile material that is used in a wide variety of applications,
from windows and
mirrors to tableware and cookware. Proper care and maintenance can help to
ensure that glass
lasts for many years. The following are some tips for caring for glass: Clean
glass regularly with
a mild soap and water solution. Avoid using abrasive cleaners or scouring
pads, as these can
scratch the surface. Dry glass wipe thoroughly after cleaning to prevent
water spots. Avoid
cleaning glass in direct sunlight, as this can cause streaks. Be careful not to
place hot objects
directly on glass, as this can cause thermal shock and breakage. If glass does
become scratched,
you can try to polish it out with a mild abrasive cleaner or a glass polishing
compound. With
proper care, glass can be a beautiful and durable addition to your home or
office. Here are some
additional tips for caring for specific types of glass: Mirrors: Mirrors should be
cleaned with a
mild soap and water solution, and then polished with a soft, lint-free
cloth. Avoid using
ammonia-based cleaners, as these can damage the mirror's reflective coating.
Tempered glass:
Tempered glass is a type of safety glass that is more resistant to
breakage than regular glass.
However, it is still important to handle tempered glass with care. Do not
clean tempered glass
with harsh chemicals or abrasive materials. Smart glass: Smart glass is a type
of glass that can
change its opacity or transparency in response to electrical signals. Smart glass
does not require
as much maintenance as regular glass, but it is important to clean it regularly
with a mild soap
and water solution
Glass is a versatile material that is used in a wide variety of applications,
from windows and
mirrors to tableware and cookware. Proper care and maintenance can help to
ensure that glass
lasts for many years. The following are some tips for caring for glass: Clean
glass regularly with
a mild soap and water solution. Avoid using abrasive cleaners or scouring
pads, as these can
scratch the surface. Dry glass wipe thoroughly after cleaning to prevent
water spots. Avoid
cleaning glass in direct sunlight, as this can cause streaks. Be careful not to
place hot objects
directly on glass, as this can cause thermal shock and breakage. If glass does
become scratched,
you can try to polish it out with a mild abrasive cleaner or a glass polishing
compound. With
proper care, glass can be a beautiful and durable addition to your home or
office. Here are some
additional tips for caring for specific types of glass: Mirrors: Mirrors should be
cleaned with a
mild soap and water solution, and then polished with a soft, lint-free
cloth. Avoid using
ammonia-based cleaners, as these can damage the mirror's reflective coating.
Tempered glass:
Tempered glass is a type of safety glass that is more resistant to
breakage than regular glass.
However, it is still important to handle tempered glass with care. Do not
clean tempered glass
with harsh chemicals or abrasive materials. Smart glass: Smart glass is a type
of glass that can
change its opacity or transparency in response to electrical signals. Smart glass
does not require
as much maintenance as regular glass, but it is important to clean it regularly
with a mild soap
and water solution
Glass is a versatile material that is used in a wide variety of applications,
from windows and
mirrors to tableware and cookware. Proper care and maintenance can help to
ensure that glass
lasts for many years. The following are some tips for caring for glass: Clean
glass regularly with
a mild soap and water solution. Avoid using abrasive cleaners or scouring
pads, as these can
scratch the surface. Dry glass wipe thoroughly after cleaning to prevent
water spots. Avoid
cleaning glass in direct sunlight, as this can cause streaks. Be careful not to
place hot objects
directly on glass, as this can cause thermal shock and breakage. If glass does
become scratched,
you can try to polish it out with a mild abrasive cleaner or a glass polishing
compound. With
proper care, glass can be a beautiful and durable addition to your home or
office. Here are some
additional tips for caring for specific types of glass: Mirrors: Mirrors should be
cleaned with a
mild soap and water solution, and then polished with a soft, lint-free
cloth. Avoid using
ammonia-based cleaners, as these can damage the mirror's reflective coating.
Tempered glass:
Tempered glass is a type of safety glass that is more resistant to
breakage than regular glass.
However, it is still important to handle tempered glass with care. Do not
clean tempered glass
with harsh chemicals or abrasive materials. Smart glass: Smart glass is a type
of glass that can
change its opacity or transparency in response to electrical signals. Smart glass
does not require
as much maintenance as regular glass, but it is important to clean it regularly
with a mild soap
and water solution
Glass is a versatile material that is used in a wide variety of applications,
from windows and
mirrors to tableware and cookware. Proper care and maintenance can help to
ensure that glass
lasts for many years. The following are some tips for caring for glass: Clean
glass regularly with
a mild soap and water solution. Avoid using abrasive cleaners or scouring
pads, as these can
scratch the surface. Dry glass wipe thoroughly after cleaning to prevent
water spots. Avoid
cleaning glass in direct sunlight, as this can cause streaks. Be careful not to
place hot objects
directly on glass, as this can cause thermal shock and breakage. If glass does
become scratched,
you can try to polish it out with a mild abrasive cleaner or a glass polishing
compound. With
proper care, glass can be a beautiful and durable addition to your home or
office. Here are some
additional tips for caring for specific types of glass: Mirrors: Mirrors should be
cleaned with a
mild soap and water solution, and then polished with a soft, lint-free
cloth. Avoid using
ammonia-based cleaners, as these can damage the mirror's reflective coating.
Tempered glass:
Tempered glass is a type of safety glass that is more resistant to
breakage than regular glass.
However, it is still important to handle tempered glass with care. Do not
clean tempered glass
with harsh chemicals or abrasive materials. Smart glass: Smart glass is a type
of glass that can
change its opacity or transparency in response to electrical signals. Smart glass
does not require
as much maintenance as regular glass, but it is important to clean it regularly
with a mild soap
and water solution
Glass is a versatile material that is used in a wide variety of applications,
from windows and
mirrors to tableware and cookware. Proper care and maintenance can help to
ensure that glass
lasts for many years. The following are some tips for caring for glass: Clean
glass regularly with
a mild soap and water solution. Avoid using abrasive cleaners or scouring
pads, as these can
scratch the surface. Dry glass wipe thoroughly after cleaning to prevent
water spots. Avoid
cleaning glass in direct sunlight, as this can cause streaks. Be careful not to
place hot objects
directly on glass, as this can cause thermal shock and breakage. If glass does
become scratched,
you can try to polish it out with a mild abrasive cleaner or a glass polishing
compound. With
proper care, glass can be a beautiful and durable addition to your home or
office. Here are some
additional tips for caring for specific types of glass: Mirrors: Mirrors should be
cleaned with a
mild soap and water solution, and then polished with a soft, lint-free
cloth. Avoid using
ammonia-based cleaners, as these can damage the mirror's reflective coating.
Tempered glass:
Tempered glass is a type of safety glass that is more resistant to
breakage than regular glass.
However, it is still important to handle tempered glass with care. Do not
clean tempered glass
with harsh chemicals or abrasive materials. Smart glass: Smart glass is a type
of glass that can
change its opacity or transparency in response to electrical signals. Smart glass
does not require
as much maintenance as regular glass, but it is important to clean it regularly
with a mild soap
and water solution
Glass is a versatile material that is used in a wide variety of applications,
from windows and
mirrors to tableware and cookware. Proper care and maintenance can help to
ensure that glass
lasts for many years. The following are some tips for caring for glass: Clean
glass regularly with
a mild soap and water solution. Avoid using abrasive cleaners or scouring
pads, as these can
scratch the surface. Dry glass wipe thoroughly after cleaning to prevent
water spots. Avoid
cleaning glass in direct sunlight, as this can cause streaks. Be careful not to
place hot objects
directly on glass, as this can cause thermal shock and breakage. If glass does
become scratched,
you can try to polish it out with a mild abrasive cleaner or a glass polishing
compound. With
proper care, glass can be a beautiful and durable addition to your home or
office. Here are some
additional tips for caring for specific types of glass: Mirrors: Mirrors should be
cleaned with a
mild soap and water solution, and then polished with a soft, lint-free
cloth. Avoid using
ammonia-based cleaners, as these can damage the mirror's reflective coating.
Tempered glass:
Tempered glass is a type of safety glass that is more resistant to
breakage than regular glass.
However, it is still important to handle tempered glass with care. Do not
clean tempered glass
with harsh chemicals or abrasive materials. Smart glass: Smart glass is a type
of glass that can
change its opacity or transparency in response to electrical signals. Smart glass
does not require
as much maintenance as regular glass, but it is important to clean it regularly
with a mild soap
and water solution
Glass is a versatile material that is used in a wide variety of applications,
from windows and
mirrors to tableware and cookware. Proper care and maintenance can help to
ensure that glass
lasts for many years. The following are some tips for caring for glass: Clean
glass regularly with
a mild soap and water solution. Avoid using abrasive cleaners or scouring
pads, as these can
scratch the surface. Dry glass wipe thoroughly after cleaning to prevent
water spots. Avoid
cleaning glass in direct sunlight, as this can cause streaks. Be careful not to
place hot objects
directly on glass, as this can cause thermal shock and breakage. If glass does
become scratched,
you can try to polish it out with a mild abrasive cleaner or a glass polishing
compound. With
proper care, glass can be a beautiful and durable addition to your home or
office. Here are some
additional tips for caring for specific types of glass: Mirrors: Mirrors should be
cleaned with a
mild soap and water solution, and then polished with a soft, lint-free
cloth. Avoid using
ammonia-based cleaners, as these can damage the mirror's reflective coating.
Tempered glass:
Tempered glass is a type of safety glass that is more resistant to
breakage than regular glass.
However, it is still important to handle tempered glass with care. Do not
clean tempered glass
with harsh chemicals or abrasive materials. Smart glass: Smart glass is a type
of glass that can
change its opacity or transparency in response to electrical signals. Smart glass
does not require
as much maintenance as regular glass, but it is important to clean it regularly
with a mild soap
and water solution

Standard Operating Procedure (SOP) For Glassware Maintenance

Generally, use the flasks, Pipettes and manifold units within 3 days of
sterilization. For Volumetric Flasks, beaker, conical flasks, test tubes, stand,
roux bottle and stopper bottle:
1. Drain out the solution from glassware completely & wash these with potable
water to remove the solution. Scrub the interior & exterior surfaces of the
glassware with 2% soap solution or 0.5% labolene using a long handle brush.
2. Drain out the soap solution and wash the glassware thoroughly with potable
water to remove the detergent completely and then rinse with purified water.
3. Dry the glassware in the oven at 60 to 65°C.
4. Plug the mouth of the dried flasks, test tubes with non-absorbent cotton plug
and wrap with Cello fen/Butter paper. Affix indicator if required.
5. Autoclave the glassware at 121°C, 15 lbs pressure for validated cycle time.
For Pipettes and burettes
1. Immediately after use dip the pipettes/burettes in freshly prepared 2% liquid
soap or 0.5% labolene solution.
2. Flush each pipettes/burettes with the jet of potable water.
3. Remove all traces of liquid soap by flushing jet of potable water thoroughly.
4. Rinse the pipettes/burettes with purified water and distilled water and dry in
an oven at 60 to 65°C.
5. Plug the mouth of the dried pipettes/burettes with non-absorbent cotton plug
6. Wrap with Cello fen/Butter paper, affix indicator if required and autoclave at
121°C, 15 lbs pressure for validated cycle time.
7. Affix labels having the date of sterilization & Use Before.
For Manifold Units
1. Disassemble the unit and wash thoroughly with 2% soap solution to gasket,
lid and interior and the exterior surface of the units.
2. Wash the unit with potable water and purified water.
3. Finally rinse the unit with distilled water and keep for drying.
4. Assemble the unit and pack with Cello fen/Butter paper Affix indicator if
required and autoclave at 121°C, 15 lbs pressure for validated cycle time.
5. Affix labels having the date of Sterilization & Use Before.
For Petri plates
1. Remove Agar from the Petri plates with a blunt knife.
2. Collect the Agar in a stainless steel container and autoclave at 121°C, 15 lbs
pressure for 30 minutes.
3. Immediately dispose the medium by burning in the specified place or drain it
in ETP.
4. Dip the plates in a washtub filled with disinfectant solution.
5. Rinse the plates with tap water and dip them in a washtub containing 2%
Soap solution or 0.5% labolene.
6. Scrub each plate with a brush or scrubber.
7. Wash each plate thoroughly with tap water to remove soap with purified
water.
8. Finally rinse with purified water. Keep the plates for drying in an oven at 60
to 65 °C.
9. After drying pack 5 to 10 Petri dishes in 1 bundle and wrap with Cello
fen/Butter paper. Affix indicator if required and autoclave at 121°C, 15 lbs
pressure for validated cycle time.
10. Affix labels having the date of Sterilization & Use Before.
Precaution
1. While handling used Petri Plates, Pipettes and Flasks, wear disposable latex
rubber hand gloves.
2. All Contaminated glass wares should be sterilized at 121°C for validated
cycle time.