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Fermentation Lab

The fermentation lab aims to observe the fermentation process using yeast (Saccharomyces) and involves setting up three conical flasks with varying combinations of warm water, sugar, and yeast. Students will monitor the size of balloons placed over the flasks to measure carbon dioxide production and record the results. The experiment concludes with a transfer of balloons to lime water to test for carbon dioxide presence and a cleanup of lab supplies.

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Nurul Shafiah Mustafa Kamal
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98 views2 pages

Fermentation Lab

The fermentation lab aims to observe the fermentation process using yeast (Saccharomyces) and involves setting up three conical flasks with varying combinations of warm water, sugar, and yeast. Students will monitor the size of balloons placed over the flasks to measure carbon dioxide production and record the results. The experiment concludes with a transfer of balloons to lime water to test for carbon dioxide presence and a cleanup of lab supplies.

Uploaded by

Nurul Shafiah Mustafa Kamal
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Fermentation Lab

Objectives: To observe the process of fermentation in a living organism, yeast (Saccharomyces).

Materials:

Conical flask, Yeast (Saccharomyces), Balloon and Sugar

Procedure:

1) Gather the needed materials. Label the 125ml conical flasks 1, 2, and 3.

Put your group’s name on each to identify your own flasks.

2) Pre

stretch the balloons.

Do this by stretching them and blowing

them up and letting out the air.

3) Set up the following flasks using the procedure for each flask.

Flask #1:

75 ml of warm water (40o C)

6.5 grams of sugar

Put the pre

stretched balloon immediately over the top of the flask

Flask #2: 75 ml of

warm water (40o C)

1 gram of yeast

Put the pre

stretched balloon immediately over the top of the flask

Flask #3: 75 ml of warm water (40o C)

6.5 grams of sugar

1 gram of yeast

Put the pre

stretched balloon immediately over the top of the flask


4) Gently stir the flask to mix the materials in each flask.

Allow the flasks to sit for some time.

5) Observe the change in the size of the balloon for each flask and record in the

table.

6) Transfer the balloons on to a flask of lime water



turn turbid in presence of carbon dioxide and record in

the table.

8) Clean up your lab supplies.

Make sure to clean out all flasks thoroughly.

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