Downloaded 17 times
Uploaded byVidya Kalaivani Rajkumar
Inoculation loop
An inoculation needle is a tool used in microbiology to transfer and inoculate microorganisms. It can be reusable, made from nichrome or platinum wire affixed to a handle, or disposable, often made from plastic resin with a blunted end. Inoculation needles are primarily used to study bacteria and fungi by streaking samples on semi-solid media or inoculating stab and slant cultures.
More Related Content
More from Vidya Kalaivani Rajkumar
Inoculation loop
- 1. Inoculation loop Aninoculation needle is laboratory equipment used in the field of microbiology to transfer and inoculate living microorganisms. It is one of the most commonly implicated biological laboratory tools and can be disposable or re-usable. A standard reusable inoculation needle is made from nichrome or platinum wire affixed to a metallic handle. A disposable inoculation needle is often made from plastic resin. The base of the needle is dulled, resulting in a blunted end. Inoculation needles are primarily applied in microbiology for studying bacteria and fungi on semi-solid media. Biotechnology, cell biology and immunology may also utilize needle-oriented culture methods. Streaking on streak plates, fish tail inoculation of slant cultures and the inoculation of stab cultures can be done with the inoculation needle. Stab cultures specifically require the inoculation needle and are used to study cell motility, microbial oxygen requirements using Thioglycolate cultures, and the gelatin liquefaction of bacteria.