BDBiosciences
Technical Specifications
BD FACSCanto II System
Innovation with Impact
Introduction
The BD FACSCanto II system delivers flow cytometry data
on an efficient, user friendly platform. Its flexible, modular
design offers automation and up to eight-color capacity for
rapid and easy production of high-quality data in clinical and
research laboratories.
Standard System
6-color 2-laser system (488, 633 nm)
Fully integrated fluidics cart
BD FACSCanto clinical software
BD FACSDiva software
System Options
8-color 3-laser system (405, 488, 633 nm)*
8-color 2-laser system (488, 633 nm)*
2D barcode reader option
Choice of two automated sample loading systems with
integrated covers for operator safety
- BD FACS Loader with 40-tube capacity
(also includes single-tube loading)
- BD High Throughput Sampler for 96- and
384-well microtiter plate capacity
Specifications
Installation Requirements
Size (D x W x H)
Cytometer 24 x 36 x 25 in.
(61 x 91 x 64 cm)
Fluidics cart 24 x 31 x 25 in.
(61 x 79 x 64 cm)
The cytometer depth increases to 30 in.
(76 cm) with BD FACS
Loader Option installed
Weight
Cytometer 320 lb (146 kg)
Fluidics cart 112 lb (51 kg)
Power
100/115/230 VAC, 50 60Hz
Operating Environment 16 30C, 20 80% non-condensing
relative humidity
Figure 1
8-peak Rainbow beads
Heat Dissipation with
BD FACS Loader installed 1843 BTU/hr
BDBiosciences
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Europe
Japan
Asia Pacific
Latin America/Caribbean
877.232.8995 888.259.0187 32.24.009.895 0120.8555.90 65.6861.0633 55.11.5185.9995
For country-specific contact information, visit bdbiosciences.com/how_to_order/
For In Vitro Diagnostic Use.
Class I (1) Laser Product.
Microsoft and Windows are registered trademarks of Microsoft Corporation.
Alexa Fluor and Texas Red are registered trademarks and Pacific Blue is a trademark of Molecular Probes, Inc., Eugene, Or.
BD, BD logo and all other trademarks are the property of Becton, Dickinson and Company. 2006 BD
* Seven- and eight-color assays on this device require validation by the user for in vitro diagnostic use.
Not validated for use with the BD Sample Prep Assistant II.
For Research Use Only. Not for use in diagnostic and therapeutic procedures.
Specifications are subject to availability and change.
PatentsPE and APC: US 4,520,110; 4,859,582; 5,055,556; Europe 76,695; Canada 1,179,942;
PerCP: US 4,876,190; Cy5.5 and Cy7: US 5,268,486; 5,486,616; 5,569,587; 5,569,766; 5,627,027;
APC-Cy7: US 5,714,386; PE-Cy7: US 4,542,104
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Optics
Lymphocytes
Monocytes
Unstained Lymphocytes
Lasers
Air-cooled:
488-nm solid state, 20-mW laser output
633-nm HeNe, 17-mW laser output
Laser Configuration
Spatially separated beams with 9 x 65 m elliptical spots
Optical Alignment
Procedure
Fixed
No operator alignment required
Flow Cell
180 x 430 m rectangular quartz flow cell
Collection Optics
Optical-gel coupled 1.2 NA lens
FSC Resolution
1.0 m
SSC Resolution
0.5 m
Fluorescence Detector
Design
Reflective optics with single transmission filter
in front of each PMT
FSC Detector
Photodiode with 488/10 BP
SSC Detector
PMT with 488/10 BP
Fluorescence Detectors
6 PMTs in 4-2 standard configuration
Blue Laser Dyes
FITC, PE, PerCP or PerCP-Cy5.5, PE-Cy7
(525, 575, 678 or 695, 785 nm)
Red Laser Dyes
APC, APC-Cy7 (660, 785 nm)
Detector Bands
Blue Laser: 530/30; 585/42; >670; 780/60 nm
Red Laser: 660/20; 780/60 nm
Fluorescence Threshold
Sensitivities
FITC <100 MESF; PE <50 MESF
Sensitivity Measurement
Using BD FACS
7-Color Setup Beads
Sensitivity determined with the setup beads measures the ability to
resolve a dimly stained population from unstained cells. This
sensitivity measure takes into account both the separation of the
populations and the broadness of the negative population.
Different fluorochromes give different separation of the stained
and unstained populations; this is also taken into account in the
sensitivity measurement. The higher the reported number, the
higher the resolution (see Figure 2).
Minimum values:
FITC >15; PE >80; PerCP >9; PerCP-Cy5.5 >25; PE-Cy7 >120;
APC >40; APC-Cy7 >16
Filter Change Procedure Keyed filters, no tools required
Fluidics
General Operation
Integrated fluidics cart and compressor with onboard housekeeping
solutions for automated startup, shutdown, and cleaning cycles
Sheath Consumption
1.10 L/hr normal operation; <1 mL/hr standby
Housekeeping Solution BD FACSFlow sheath solution 20 L
Capacities
BD FACSClean solution 5 L
BD FACS shutdown solution 5 L
Waste tank 10 L
Carryover
0.1%
Sample Injection
Direct into flow cell
Max Particle Size
50 m
Sample Flow Rate, Min 10 L/min
Sample Flow Rate, Max 120 L/min
Figure 2
Lysed whole blood gated on lymphocytes.
Cytometer set up with sensitivity from
BD FACS 7-color setup beads
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Sample Acquisition Rate 10,000 events/sec, 6 compensated fluorescent parameters and 2
scatter parameters
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Sample Dead Volume
30 L
(BD Falcon tubes 12 x 75 mm)
System Cleaning
Daily: Automated startup and shutdown procedures
Monthly: Run long clean
Data Management System
Parameters
Area (A), Width (W), Height (H) for all channels with up to
2 ratios, and Time (T)
Signal Processing
18-bit dynamic range with IEEE 32-bit floating-point resolution
Threshold
Single parameter (any channel) or logical combinations of multiple
parameters (any or all channels)
Compensation
Full inter-beam matrix, during or post acquisition
Maximum Logical Gate
Regions
Limited only by system memory (2 GB RAM)
CPU/Monitors
HP Xw4300, with either 19" or 24" flat screen monitors
Software
BD FACSDiva v5.0.1, BD FACSCanto clinical v2.1
Operating System
Microsoft Windows XP Pro
Cytometer Options
8-Color Option with 3 Lasers
Lasers
Air-cooled:
405-nm solid state diode, 30-mW fiber power output
488-nm solid state, 20-mW laser output
633-nm HeNe, 17-mW laser output
Fluorescence Detectors
8 PMTs in 4-2-2 configuration
Laser Dyes
Violet: Pacific Blue, AmCyan (455, 488 nm)
Blue: FITC, PE, PerCP or PerCP-Cy5.5, PE-Cy7
(525, 575, 678, or 695, 785 nm)
Red: APC, APC-Cy7 (660, 785 nm)
Detector Bands
Violet: 450/50; 502 to 525 nm
Blue: 530/30; 585/42; >670; 780/60 nm
Red: 660/20; 780/60 nm
8-Color Option with 2 Lasers
Lasers
Air-cooled:
488-nm solid state, 20-mW output
633-nm HeNe, 17-mW output
Fluorescence Detectors
8 PMTs in 5-3 configuration
Laser Dyes
Blue: FITC, PE, PE-Texas Red, PerCP or PerCP-Cy5.5, PE-Cy7
(525, 575, 615, 678 or 695, 785 nm)
Red: APC, Alexa Fluor 700, APC-Cy7
(660, 720, 785 nm)
Detector Bands
Blue: 530/30; 585/42; 616/23; >670; 780/60 nm
Red: 660/20; 712/21; 780/60 nm
Figure 3
Lysed whole human blood stained with
CD4 antibody conjugates
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Cytometer Options
(continued)
Sample Input with BD FACS Loader
Loading
40-tube carousel
Sample/test ID
Indexed carousel, with carousel ID barcode reader
Worklist importable from BD FACS Sample Prep Assistant II
Throughput
56 min/carousel with BD Multi-Check high controls,
66 min/carousel with BD Multi-Check low controls
using BD Multitest 6-color TBNK application
Miscellaneous
Multiple clinical applications can be run on the same Loader carousel.
Sample Input with BD High Throughput Sampler
(see BD HTS Specification Sheet for more details)
Loading
96- and 384-well microtiter plates
Throughput
<15 min/96-well plate in high-throughput mode with 2-sec
acquisition
Carryover
1%
Barcode Reader with Stand
Figure 4
CENs stained with propidium iodide (top) and
Hoechst 33258 dye (bottom)
Use with
BD FACSCanto clinical software
2D Reader
Streamlined input of BD FACS 7-color setup bead target values,
input of patient information
Figure 5
E. coli bacterium. Pecan pollen (45 to 55 m).
K562 cells.
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