Project #: Tit
Author: Remington Musick
Due Date: February 22,20221
Lab Day & Time: Monday, 9 A.M.
Introduction (3 pts)
In this lab we were performing a simple stain on bacterial colonies that were collected the week
prior. The significance of this exercise is to be able to collect and incubate microbes in order to
stain them for observation. Which allows you to be able to effectively see what shape and structure
the bacteria on your skins looks like. What I hope to gain from this lab is to be able to successfully
stain a slide then be able to identify the shape and structure of some bacteria on the slide. Which
will be accomplished by staining the bacterial colonies to observe under the microscope.
Hypothesis (2 pt)
If the bacterial sample that was collected the week prior grew in the incubator, then i will be able to
stain a sample to look under the microscope. If the staining process is done correctly then I should
be able to clearly see the shape, and structure of the bacteria collected.
Materials and Methods (2 pt)
Materials:
1. Bacteria Plate
2. Slide
3. Inoculating Loop
4. Bunsen Burner
5. Glass Slide
6. Slide Holder
7. Crystal Violet Stain
8. Stain Disposal Tray
9. Blotting Paper
10. Microscope
11. Oil Immersion
Methods: 1. First we took the bacterial plate, with the inoculating loop dipped it in water and placed
that water on the slide. 2.Collected a sample of the bacteria and gently smeared it on the slide. 3.
Allowed the smear to air dry until appeared opaque and foggy. 4. Using slide holder we heat fixed
the slide by running it through a bunsen burner 2-3 times. 5 Allowed the slide to cool down before
starting the staining process. 6. Taking the crystal violet stain we covered the heat fixed bacterial
with the stain and left it to dry for one minute. 7. Once the slide was left for one minute covered in
the crystal violet stain it was rinsed off using water. 8. Then fired using blotting paper. 9. Once the
slide has successfully been stained we looked at it under the microscope starting at the lowest
resolution and eventually making our way to 100X and used Oil Immersion to allow for a more clear
picture. 10. Examined what was under the microscope and describes its shape, and it’s structure.
Results (3 pts)
After collecting, staining, and viewing the bacteria under the microscope we were able to physically
see and differentiate between staphylococcus and streptococcus. As seen the simple stain under
Image: Crystal violet bacteria stain under 400x
400X. The arrow on the upper right hand side of the photo highlights bacteria with a
staphylococcus shape and structure. This distinction between staphylococcus and streptococcus
was made due to the bacteria having a cluster shape instead of a chain-like structure.
Image: Normal Flora plate
The sample was removed from this plate, where the simple of my bacterial skin was placed on the
plate and incubated until used.
Analysis (3 pts)
Based on the results of the experiment, the hypothesis was shown to be correct. Since the staining
process was done correctly We were able to visibly and clearly see the shape and structure of the
bacteria that was stained.
Conclusion (2 pts)
Based on the results of the lab I have gained the skill of correctly staining bacteria using crystal
violet stain. The results answered the question by being able to see exactly the shape and
structure of the bacteria on the slide. Crystal violet shard appeared on the slide under the
microscope, to prevent this from happening a second time, when rinsing the slide of the stain, I
need to make sure to wash it more cleaner. This should
hopefully remove the shards of crystal Violet to insure a more clear and uninterrupted view of the
bacteria.
